Coding

Part:BBa_K2271141:Design

Designed by: Manuel Lentzen   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)


Pex13 membrane anchor with mRuby


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 1980
    Illegal BglII site found at 879
    Illegal BglII site found at 916
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2335
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The membrane domain of Pex13 was desinged as a peroxisomal membrane anchor for proteins as previously described by Erdmann et al. (2004). We only used the amino acids 200-310 and added a triple GGGGS linker to allow an undisturbed c-terminal fusion of any protein of interest.

Source

UniProtKB - P80667 (PEX13_YEAST)

References

Peroxisomal Membrane Proteins Contain Common Pex19p-binding Sites that Are an Integral Part of Their Targeting Signals - Erdmann et al., 2004